研究生教学

-基本信息

-研究兴趣及简介

微流控生物分析、体外诊断、分子病理、器官芯片

主要以复杂生物体系为研究对象，建立靶标富集新策略，发展信号转化与放大新方法，从而实现生物体系中痕量物质的高灵敏、高选择性检测。目前已主持国家自然科学基金（面上、青年）、福建省自然科学基金、国家重点实验室开放基金、校企产学研项目、泉州市科技计划项目、中青年教师科技创新计划等课题。以第一/通讯作者在ACS Nano、Angew Chem Int Ed、Lab Chip、Anal Chem、ACS Appl Mater Inter、Biosens Bioelectron、Sens Actuators等期刊发表SCI论文20余篇。获评福建省高层次人才、厦门市高层次人才、泉州市高层次人才、参与获得厦门市科技进步一等奖。

-教育经历

博士（厦门大学化学生物学专业2014-2017）

硕士（湖南大学生物化学与分子生物学专业2007-2010）

学士（贵州大学生物科学专业2003-2007）

-研究工作经历

副教授（华侨大学 2020.12-至今）

讲师（华侨大学 2018-2020）

助理研究员（厦门大学附属成功医院 2017-2018）

研究实习员、助理研究员（厦门大学附属成功医院 2010-2014）

-主要代表论文

1) Huang Ruolan; Liu Kun; Yue Chensirong; Wang Ying; Zeng Zhilong; Xiao Lu; Dong Lei^*; Ke Rongqin^*; Yang Chaoyong^*; Liu Dan^*. Chromogenic visualization of single RNA molecules in situ with duplex capability by rolling circle amplification with alkaline phosphatase. Analytical Chemistry, 2023, 95(32), 12161-12168.

2) Wu Qiuyue; Zhu Lin; Wei Xinyu; Zhang Mingxia; Zhang Chi; You Zhenlong; Zhang Suhui; Song Yanling^*; Liu Dan^*; Yang Chaoyong^*. Detection of circulating tumor cells using antibody-functionalized microchips to monitor tumorigenesis in a mouse model of metastatic breast cancer. Sensors and Actuators B: Chemical, 2022, 133274.

3) Ling Jiajun^#; Liu Dan^#; Zhang Jialu #; Zhu Lin; Wan Shuang; Yang Chaoyong; Song Yanling^*. Thermodynamic and Kinetic Modulation of Microfluidic Interfaces by DNA Nanoassembly Mediated Merit-Complementary Heteromultivalency. ACS Nano, 2022, 16:20915–20921.

4) Liu Dan^*; Zhu Mingyang; Lin Yi; Li Mengmeng; Huang Ruolan; Yang Liu^*; Song Yanling; Diao Yong; Yang Chaoyong^*. LY6E protein facilitates adeno-associated virus crossing in a biomimetic chip model of the human blood-brain barrier. Lab on a Chip, 2022, 22: 4180-4190.

5) Liu, Dan^*; Wang, Ying; Li, Mengmeng; Wu, Qiaoyi^*; Song, Yanling; Zhu, Zhi; Yang, Chaoyong^*; Integrated microfluidic devices for invitro diagnostics at point of care. Aggregate, 2022, DOI:10.1002/agt2.184.

6) Liu, Dan^*; Shen, Haicong; Zhang, Yuqian; Shen, Danyu; Zhu, Mingyang; Song, Yanling; Zhu, Zhi; Yang, Chaoyong^*; A microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay for rapid COVID-19 detection. Lab on a Chip, 2021, 21: 2019-2026.

7) Liu, Dan; Zhang, Yuqian; Zhu, Mingyang; Yu, Zongzhong; Ma, Xiaoming; Song, Yanling; Zhou, Shufeng^*; Yang, Chaoyong^*; Microfluidic-integrated multicolor immunosensor for visual detection of HIV-1 p24 antigen with the naked eye, Analytical Chemistry, 2020, 92 (17):11826-11833.

8) Liu Dan^*; Zhu, Mingyang; Zhang, Yuqian; Diao, Yong; Crossing the blood-brain barrier with AAV vectors. Metabolic Brain Disease, 2021, 36 (1), 45-52.

9) Liu Dan^*; Wang Junxia; Wu Lingling; Huang Yishun; Zhang Yuqian; Zhu Mingyang; Wang Yang; Zhu Zhi^*; Yang Chaoyong; Trends in miniaturized biosensors for point-of-care testing, TrAC Trends in Analytical Chemistry, 2020,122: 115701.

10) Liu Dan^*; Liu Fang; Huang Yishun; Song Yanling; Zhu Zhi; Zhou Shu-feng; Yang Chaoyong^*; Catalase-linked immunosorbent pressure assay for portable quantitative analysis, Analyst, 2019, 144 (14): 4188-4193.

11) Liu Dan; Tian Tian; Chen Xiaofeng; Lei Zhichao; Song Yanling; Shi Yuanzhi; Ji Tianhai; Zhu Zhi; Yang Liu; Yang Chaoyong^*; Gas-generating reactions for point-of-care testing, Analyst, 2018, 143 (6): 1294-1304.

12) Tao Qian; Wu Xinyi; Lin Qingyuan; Zheng Haiyan; Yang Wensheng; Liu Dan^*; Yang Chaoyong James; Ji Tianhai^*; Portable detection of serum HER-2 in breast cancer by a pressure-based platform, Analytical and bioanalytical chemistry, 2018, 410 (28): 7489-7498.

13) Liu Dan; Li Xingrui; Zhou Junkai; Liu Shibo; Tian Tian; Song Yanling; Zhu Zhi; Zhou Leiji; Ji Tianhai; Yang Chaoyong^*; A fully integrated distance readout ELISA-Chip for point-of-care testing with sample-in-answer-out capability, Biosensors and Bioelectronics, 2017, 96: 332-338.

14) Liu Dan; Jia Shasha; Zhang Huimin; Ma Yanli; Guan Zhichao; Li Jiuxing; Zhu Zhi^*; Ji Tianhai; Yang Chaoyong James^*; Integrating target-responsive hydrogel with pressuremeter readout enables simple, sensitive, user-friendly, quantitative point-of-care testing, ACS Applied Materials & Interfaces, 2017, 9 (27): 22252-22258.

15) Ji Tianhai^#; Liu Dan^#; Liu Fang; Li Jiuxing; Ruan Qingyu; Song Yanling; Tian Tian; Zhu Zhi; Zhou Leiji; Lin Hui^*; Chaoyong Yang; Dong Wang^*; A pressure-based bioassay for the rapid, portable and quantitative detection of C-reactive protein, Chemical Communications, 2016, 52 (54): 8452-8454.

16) Zhu Zhi^#; Guan Zhichao^#; Liu Dan^#; Jia Shasha^#; Li Jiuxing; Lei Zhichao; Lin Shuichao; Ji Tianhai; Tian Zhongqun; Yang Chaoyong James^*; Translating molecular recognition into a pressure signal to enable rapid, sensitive, and portable biomedical analysis, Angewandte Chemie International Edition, 2015, 54 (36): 10448-10453.

17) Liu Dan; Lin Bingqian; Shao Wei; Zhu Zhi; Ji Tianhai^*; Yang Chaoyong^*; In vitro and in vivo studies on the transport of PEGylated silica nanoparticles across the blood–brain barrier, ACS applied materials & interfaces, 2014, 6 (3): 2131-2136.

18) Liu Dan; He Xiaoxiao^*; Wang Kemin^*; He Chunmei; Shi Hui; Jian Lixin; Biocompatible silica nanoparticles−insulin conjugates for mesenchymal stem cell adipogenic differentiation, Bioconjugate chemistry, 2010, 21 (9): 1673-1684.

-授权发明专利

1) 刘丹; 杨朝勇; 刁勇; 张语倩; 沈海聪; 成志云; 用于检测COVID-19病毒的RT-RAA引物对和诊断试剂盒, ZL202010578160.X

2) 杨朝勇; 刘丹; 沈海聪; 张语倩; 李博安; 核酸检测的方法及其装置和在COVID-19检测中的应用, ZL202010578157.8